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Hepatobiliary Pancreat Dis Int. 2003 Aug;2(3):467-70.

Analysis of gene expression profiles in pancreatic carcinoma by using cDNA microarray.

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1
Center for Pancreatic Cancer, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China. yuxianjun@hotmail.com

Abstract

OBJECTIVES:

To survey the gene expression profiles in pancreatic carcinoma by using cDNA microarray and detect target genes for further study.

METHODS:

Three mixed samples from 2 cases of normal pancreatic tissue and 4 cases of moderate-differentiated pancreatic carcinoma were studied by means of cDNA microarray consisting of 18 000 genes.

RESULTS:

1484 and 1353 different expressed genes were observed in two cancer samples respectively. We identified 455 genes altered with the same tendency in both samples, including 102 up-regulated and 353 down-regulated genes. There were 274 known genes and 181 unknown genes; 27.8% and 52.0% genes respectively had an expression level in cancer that was 2-fold higher or lower than that in normal samples. Tumor suppressor genes, growth factors and receptor genes, signal conduction genes, transcription factor genes were identified.

CONCLUSIONS:

cDNA microarray is an efficient and high-throughout method to investigate gene expression profiles in pancreatic carcinoma. MBD1, EDG1 and gene hypermethylation mechanism would play an important role in the pathogenesis of pancreatic carcinoma.

PMID:
14599962
[Indexed for MEDLINE]
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