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J Electron Microsc (Tokyo). 2003;52(4):429-33.

Comparison of hexamethyldisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge.

Author information

1
Departamento de Hidráulica e Saneamento, Universidade de São Paulo, Escola de Engenharia de São Carlos, Av. Trabalhador São Carlense 400, São Carlos-SP, 13566-590, Brazil. juli.calabria@ig.com.br

Abstract

We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.

PMID:
14599106
DOI:
10.1093/jmicro/52.4.429
[Indexed for MEDLINE]

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