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FEBS Lett. 2003 Nov 6;554(1-2):184-8.

Spectral tuning of obelin bioluminescence by mutations of Trp92.

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  • 1Photobiology Lab, Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk 660036, Russia.


The Ca(2+)-regulated photoprotein obelin was substituted at Trp92 by His, Lys, Glu, and Arg. All mutants fold into stable conformations and produce bimodal bioluminescence spectra with enhanced contribution from a violet emission. The W92R mutant has an almost monomodal bioluminescence (lambdamax=390 nm) and monomodal fluorescence (lambdamax=425 nm) of the product. Results are interpreted by an excited state proton transfer mechanism involving the substituent side group and His22 in the binding cavity.

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