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Appl Opt. 2003 Oct 20;42(30):6184-91.

Detection of bacteria by time-resolved laser-induced breakdown spectroscopy.

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Direction Generale de l'Armement, Direction des Centres d'expertises et d'essais, Centre d'Etudes du Bouchet, B.P. 91710 Vert-Le-Petit, France.


A laser-induced breakdown spectroscopy technique for analyzing biological matter for the detection of biological hazards is investigated. Eight species were considered in our experiment: six bacteria and two pollens in pellet form. The experimental setup is described, then a cumulative intensity ratio is proposed as a quantitative criterion because of its linearity and reproducibility. Time-resolved laser-induced breakdown spectroscopy (TRELIBS) exhibits a good ability to differentiate among all these species, whatever the culture medium, the species or the strain. Thus we expect that TRELIBS will be a good candidate for a sensor of hazards either on surfaces or in ambient air.

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