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FEMS Microbiol Lett. 1992 Nov 1;77(1-3):161-8.

Analysis of genomic DNA from Chlamydia trachomatis for Dam and Dcm methylation.

Author information

1
Department of Pathology and Laboratory Medicine, University of California, Los Angeles.

Abstract

Chlamydia trachomatis is a Gram-negative eubacterium with a dimorphic developmental cycle and obligate intracellular growth in the eucaryotic host. The Dam transmethylase of Escherichia coli methylates at the N6 position of adenine in the sequence 5'-GATC-3' and the Dcm transmethylase adds methyl groups to the C5 position of the internal cytosines in the sequences 5'-CCWGG-3'. In contrast to E. coli, C. trachomatis DNA appears to have unmethylated Dam sites and only low level Dcm methylation.

PMID:
1459404
DOI:
10.1016/0378-1097(92)90149-i
[Indexed for MEDLINE]

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