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Science. 2003 Oct 31;302(5646):842-6.

Empirical analysis of transcriptional activity in the Arabidopsis genome.

Author information

1
Plant Gene Expression Center, Albany, CA 94710, USA.

Abstract

Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.

PMID:
14593172
DOI:
10.1126/science.1088305
[Indexed for MEDLINE]
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