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Opt Lett. 2003 Oct 15;28(20):1918-20.

Multiphoton multifocal microscopy exploiting a diffractive optical element.

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University of Trento, Via Sommarive 14, 38050 Povo, Trento, Italy.


Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds.


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