Abstract
This paper focuses on the development of candidate DNA vaccine encoding antigenic epitopes of type O foot-and-mouth disease virus (FMDV). A series of plasmids encoding different combinations of B cell epitopes and a T cell epitope were constructed and characterized by inoculating BALB/c mice. The specific antibodies were only detectable in the mice inoculated with plasmids encoding the T cell epitope and B cell epitopes from sites 5 and 1, within which site 5 includes residues 135-167 of VP1 and site 1 includes 141-160 region (G-H loop) and carboxyl terminus of VP1. Stronger cellular immune responses were also observed in these mice using T cell proliferation assay.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Electrophoresis, Polyacrylamide Gel
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Epitopes, B-Lymphocyte / genetics*
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Epitopes, B-Lymphocyte / immunology
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Epitopes, T-Lymphocyte / genetics*
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Epitopes, T-Lymphocyte / immunology
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Female
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Foot-and-Mouth Disease / immunology
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Foot-and-Mouth Disease / prevention & control*
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Foot-and-Mouth Disease / virology
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Foot-and-Mouth Disease Virus / immunology*
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Immunoblotting
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Injections, Intramuscular
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Mice
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Mice, Inbred BALB C
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Plasmids
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Vaccination
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Vaccines, DNA / immunology*
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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Epitopes, B-Lymphocyte
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Epitopes, T-Lymphocyte
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Vaccines, DNA
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Viral Vaccines