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Gene. 2003 Oct 30;318:75-81.

Differential expression of PE and PE_PGRS genes in Mycobacterium tuberculosis strains.

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1
Departamento de Inmunologi;a, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, México, D.F. 04510, Mexico.

Abstract

In this study, the expression of the Mycobacterium tuberculosis PE, PE_polimorphic GC-rich sequences (PGRS) gene family encoding approximately 99 glycine-rich proteins was assayed by reverse-transcriptase polymerase chain reaction (RT-PCR) in M. tuberculosis H37Rv, Mycobacterium canettii and two clinical isolates of M. tuberculosis. Restriction analyses and sequencing of the RT-PCR products showed that all the strains expressed the PE Rv1172c gene while the PE_PGRS Rv3652 gene was only expressed by one of the M. tuberculosis clinical isolates, and the PE_PGRS Rv0578c was not expressed by M. canettii. It was also determined that the PE_PGRS Rv0278c and Rv0279c were not expressed by any of the studied strains. The data presented in this report show that the PE, PE_PGRS genes are differentially expressed in M. tuberculosis strains during in vitro growth. These findings suggest that PE, PE_PGRS genes may play a role in protein variation between M. tuberculosis strains.

PMID:
14585500
[Indexed for MEDLINE]

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