Format

Send to

Choose Destination
See comment in PubMed Commons below
Jpn J Infect Dis. 2003 Aug;56(4):151-5.

Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.

Author information

1
Department of Clinical Laboratory Science, College of Health Sciences, Korea University, Seoul 136-703, Korea.

Abstract

A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identification of S. enterica serovar Typhimurium.

PMID:
14583637
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for National Institute of Infectious Diseases
    Loading ...
    Support Center