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J Agric Food Chem. 2003 Nov 5;51(23):6657-62.

Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products.

Author information

1
Cereal Quality Lab, Soil & Crop Sciences Department, Texas A&M University, College Station, Texas 77843, USA. jawika@tamu.edu

Abstract

Specialty sorghums, their brans, and baked and extruded products were analyzed for antioxidant activity using three methods: oxygen radical absorbance capacity (ORAC), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). All sorghum samples were also analyzed for phenolic contents. Both ABTS and DPPH correlated highly with ORAC (R(2) = 0.99 and 0.97, respectively, n = 18). Phenol contents of the sorghums correlated highly with their antioxidant activity measured by the three methods (R(2) >or= 0.96). The ABTS and DPPH methods, which are more cost effective and simpler, were demonstrated to have similar predictive power as ORAC on sorghum antioxidant activity. There is a need to standardize these methods to allow for data comparisons across laboratories.

PMID:
14582956
DOI:
10.1021/jf034790i
[Indexed for MEDLINE]

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