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Curr Opin Chem Biol. 2003 Oct;7(5):635-40.

Analysis of protein interactions using fluorescence technologies.

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  • 1Department of Physiology, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA.


Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.

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