Simultaneous determination of tryptophan and glutathione in individual rat hepatocytes by capillary zone electrophoresis with electrochemical detection at a carbon fiber bundle--Au/Hg dual electrode

Anal Chem. 2003 Aug 1;75(15):3859-64. doi: 10.1021/ac0207022.

Abstract

A method for single-cell analysis was developed by combining capillary zone electrophoresis (CZE) with electrochemical detection (ECD) using a dual electrode consisting of two different types of electrode material (carbon fiber and Au/Hg). In this method, the parallel mode was used. Different potentials were applied to both electrodes of the dual electrode. Tryptophan and glutathione, which could not be simultaneously detected by normal CZE-ECD, could be simultaneously and selectively detected by CZE-ECD at the dual electrode in one run, respectively. The CZE-ECD system with the dual electrode was applied to determine them in individual rat hepatocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon / chemistry*
  • Carbon Fiber
  • Catecholamines / chemistry
  • Catecholamines / metabolism
  • Electrochemistry
  • Electrodes
  • Electrophoresis, Capillary / instrumentation
  • Electrophoresis, Capillary / methods*
  • Glutathione / analysis*
  • Glutathione / metabolism
  • Gold / chemistry*
  • Hepatocytes / chemistry*
  • Mercury / chemistry*
  • Rats
  • Serotonin / chemistry
  • Serotonin / metabolism
  • Tryptophan / analysis*
  • Tryptophan / metabolism

Substances

  • Carbon Fiber
  • Catecholamines
  • Serotonin
  • Carbon
  • Gold
  • Tryptophan
  • Mercury
  • Glutathione