Format

Send to

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12724-8. Epub 2003 Oct 20.

Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones.

Author information

1
Department of Physics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 12-111, Cambridge, MA 02139, USA. kch23@mit.edu

Abstract

In Escherichia coli, division site selection is regulated in part by the Min-protein system. Oscillations of the Min proteins from pole to pole every approximately 40 sec have been revealed by in vivo studies of GFP fusions. The dynamic oscillatory structures produced by the Min proteins, including a ring of MinE protein, compact polar zones of MinD, and zebra-striped oscillations in filamentous cells, remain unexplained. We show that the Min oscillations, including mutant phenotypes, can be accounted for by in vitro-observed interactions involving MinD and MinE, with a crucial role played by the rate of nucleotide exchange. Recent discoveries suggest that protein oscillations may play a general role in proper chromosome and plasmid partitioning.

PMID:
14569005
PMCID:
PMC240685
DOI:
10.1073/pnas.2135445100
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center