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J Inorg Biochem. 2003 Dec 1;97(4):384-7.

Fluorescent complex of pyoverdin with aluminum.

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1
Departamento de NutriciĆ³n y Bromatologi;a III, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040, Madrid, Spain.

Abstract

When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.

PMID:
14568244
[Indexed for MEDLINE]
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