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Phytochemistry. 2003 Nov;64(6):1069-76.

Cloning and regiospecificity studies of two flavonoid glucosyltransferases from Allium cepa.

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1
Syngenta Biotechnology Incorporated, 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA.

Abstract

Two UDP-glucose-dependent flavonoid glucosyltransferases (EC 2.4.1.-) isolated from the epidermal layer of yellow onion (Allium cepa) were functionally expressed in Escherichia coli and their substrate specificity investigated. The two enzymes exhibited different substrate- and regio-specificity profiles. A. cepa UGT73G1 used a wide range of different flavonoid substrates including flavonoids not naturally occurring in onion. Regiospecificity was indicated for hydroxyl-groups of the C-3, C-7 and C-4' positions of the flavan backbone structure to yield flavonoid mono- and diglucosides. In contrast, A. cepa UGT73J1 showed activity only with the flavonoid mono-glucoside isoquercitrin and the isoflavone aglycone genistein, with regiospecificity for the C-7 position. The regiospecificity for both enzymes included positions that are glucosylated in flavonoids of onion bulbs, indicating their involvement in flavonoid biosynthesis in A. cepa.

PMID:
14568073
DOI:
10.1016/s0031-9422(03)00507-7
[Indexed for MEDLINE]

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