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Nat Biotechnol. 2003 Nov;21(11):1303-4. Epub 2003 Oct 19.

Immunofluorescence stimulated emission depletion microscopy.

Author information

1
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Göttingen, Germany.

Abstract

We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.

PMID:
14566345
DOI:
10.1038/nbt897
[Indexed for MEDLINE]

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