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Mol Biol Cell. 2004 Jan;15(1):37-45. Epub 2003 Oct 17.

Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP.

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1
Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA.

Abstract

The centrosome in animal cells provides a major microtubule-nucleating site that regulates the microtubule cytoskeleton temporally and spatially throughout the cell cycle. We report the identification in Drosophila melanogaster of a large coiled-coil centrosome protein that can bind to calmodulin. Biochemical studies reveal that this novel Drosophila centrosome protein, centrosome protein of 309 kDa (CP309), cofractionates with the gamma-tubulin ring complex and the centrosome-complementing activity. We show that CP309 is required for microtubule nucleation mediated by centrosomes and that it interacts with the gamma-tubulin small complex. These findings suggest that the microtubule-nucleating activity of the centrosome requires the function of CP309.

PMID:
14565985
PMCID:
PMC307525
DOI:
10.1091/mbc.E03-03-0191
[Indexed for MEDLINE]
Free PMC Article
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