Format

Send to

Choose Destination
Theor Appl Genet. 2004 Feb;108(3):558-66. Epub 2003 Oct 16.

Comparative mapping between quercus and castanea using simple-sequence repeats (SSRs).

Author information

1
INRA, UMR Biodiversité Gènes et Ecosystèmes, 69 Route d'Arcachon, 33612, Cestas Cedex, Pierroton, France.

Abstract

Simple sequence repeat (SSR) markers from Quercus and Castanea were used for comparative mapping between Quercus robur (L.) and Castanea sativa (Mill.). We tested the transferability of SSRs developed in Quercus to Castanea and vice-versa. In total, 47% (25) of the Quercus SSRs and 63% (19) of the Castanea SSRs showed a strong amplification product in the non-source species. From these 44 putative comparative anchor tags, 19 (15 from Quercus and 4 from Castanea) were integrated in two previously established genetic linkage maps for the two genera. SSR loci were sequenced to confirm the orthology of the markers. The combined information from both genetic mapping and sequence analysis were used to determine the homeology between seven linkage groups, aligned on the basis of pairs or triplets of common markers, while two additional groups were matched using a single microsatellite marker. Orthologous loci identified between Q. robur and C. sativa will be useful as anchor loci for comparative mapping studies within the Fagaceae family.

PMID:
14564395
DOI:
10.1007/s00122-003-1462-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center