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Nat Cell Biol. 2003 Sep;Suppl:S14-9.

4D imaging to assay complex dynamics in live specimens.

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Gene Expression and Cell Biology/Biophysics Programmes, European Molecular Biology Laboratory, Heidelberg, Germany.


A full understanding of cellular dynamics is often difficult to obtain from time-lapse microscopy of single optical sections. New microscopes and image-processing software are now making it possible to rapidly record three-dimensional images over time. This four-dimensional imaging allows precise quantitative analysis and enhances visual exploration of data by allowing cellular structures to be interactively displayed from many angles. It has become a key tool for understanding the complex organization of biological processes in live specimens.

[Indexed for MEDLINE]

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