Abstract
In this study, we have crystallized class II fructose-1,6-bisphosphate aldolase (FBA) from Thermus caldophilus (Tca). Purified Tca FBA is a tetrameric enzyme of 305 residues, which crystallizes in the space group P2(1)2(1)2(1) (cell dimensions a = 98.9, b = 113.1, c = 115.7 A), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 A.
MeSH terms
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Amino Acid Sequence
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Crystallization
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Crystallography, X-Ray / statistics & numerical data
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fructose-Bisphosphate Aldolase / chemistry*
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Fructose-Bisphosphate Aldolase / genetics
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Fructose-Bisphosphate Aldolase / isolation & purification
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Molecular Sequence Data
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Recombinant Proteins / chemistry
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Sequence Alignment
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Thermus / enzymology*
Substances
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Recombinant Proteins
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Fructose-Bisphosphate Aldolase