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Protein Pept Lett. 2003 Oct;10(5):459-68.

Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch hydrolysis.

Author information

1
Western Regional Research Center, USDA-Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA. dwsw@pw.usda.gov

Abstract

High-throughput screening for high-activity barley alpha-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed colorimetric and chemiluminescent detection systems that enable direct and rapid screening of activities on raw starch substrate. Active clones could be separated into two groups, based on high total activity or high specific activity.

PMID:
14561134
DOI:
10.2174/0929866033478744
[Indexed for MEDLINE]

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