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Genomics. 2003 Nov;82(5):580-2.

A simple and cost-effective method for rapid genotyping of insertion/deletion polymorphisms.

Author information

1
Coriell Institute for Medical Research, Camden, NJ 08103, USA. rrobledo@coriell.umdnj.edu

Abstract

We developed a simple method, based on the TaqMan technology, for fast genotyping of insertion/deletion polymorphisms of known location. The genotypes of 22 CEPH individuals, previously ascertained by conventional methods, were confirmed in the new assay without manual, time-consuming, post-PCR analysis. We propose to expand the application of TaqMan probes for population screening of insertion/deletion polymorphisms in which the exact endpoints of the insertion/deletion are known. The method can be applied to polymorphisms of any size and can be used for different applications such as diagnostics, genome variation, and species identification.

PMID:
14559216
DOI:
10.1016/s0888-7543(03)00172-1
[Indexed for MEDLINE]

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