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Nat Cell Biol. 2003 Nov;5(11):967-71. Epub 2003 Oct 12.

EB1 reveals mobile microtubule nucleation sites in Arabidopsis.

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Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich, NR4 7UH, UK.

Erratum in

  • Nat Cell Biol. 2004 Jan;6(1):77.


In plants, it is unclear how dispersed cortical microtubules are nucleated, polarized and organized in the absence of centrosomes. In Arabidopsis thaliana cells, expression of a fusion between the microtubule-end-binding protein AtEB1a and green fluorescent protein (GFP) results in labelling of spindle poles, where minus ends gather. During interphase, AtEB1a-GFP labels the microtubule plus end as a comet, but also marks the minus end as a site from which microtubules can grow and shrink. These minus-end nucleation sites are mobile, explaining how the cortical array can redistribute during the cell cycle and supporting the idea of a flexible centrosome in plants.

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