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J Virol. 2003 Nov;77(21):11846-8.

Binding of transmissible gastroenteritis coronavirus to brush border membrane sialoglycoproteins.

Author information

  • 1Institut für Virologie, Tierärztliche Hochschule Hannover, 30559 Hannover, Germany. Christel.Schwegmann@tiho-hannover.de

Abstract

Transmissible gastroenteritis coronavirus (TGEV) is a porcine pathogen causing enteric infections that are lethal for suckling piglets. The enterotropism of TGEV is connected with the sialic acid binding activity of the viral surface protein S. Here we show that, among porcine intestinal brush border membrane proteins, TGEV recognizes a mucin-type glycoprotein designated MGP in a sialic acid-dependent fashion. Virus binding assays with cryosections of the small intestine from a suckling piglet revealed the binding of TGEV to mucin-producing goblet cells. A nonenteropathogenic mutant virus that lacked a sialic acid binding activity was unable to bind to MGP and to attach to goblet cells. Our results suggest a role of MGP in the enteropathogenicity of TGEV.

PMID:
14557669
PMCID:
PMC229351
[PubMed - indexed for MEDLINE]
Free PMC Article
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