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Biochem Biophys Res Commun. 2003 Oct 24;310(3):943-8.

Improved methods for the acquisition and interpretation of NMR metabolomic data.

Author information

1
Department of Environmental Toxicology, University of California, Davis, CA 95616, USA. MRViant@UCDavis.edu

Abstract

The high spectral congestion typically observed in one-dimensional (1D) 1H nuclear magnetic resonance (NMR) spectra of tissue extracts and biofluids limits the metabolic information that can be extracted. This study evaluates the application of two-dimensional J-resolved (JRES) spectroscopy for metabolomics, which can provide proton-decoupled projected 1D spectra (p-JRES). This approach is illustrated by an investigation of embryogenesis in Japanese medaka (Oryzias latipes), an established fish model for developmental toxicology. When combined with optimized spectral pre-processing,(2) including a 0.005-ppm bin width for data segmentation and a logarithmic transformation, the reduced congestion in the p-JRES spectra increases the likelihood that a specific metabolite can be accurately integrated and thus increases the extractable information content of the spectra. Principal components analysis of the p-JRES spectra reveals the concept of a developmental trajectory that summarizes the changes in the NMR-visible metabolome throughout medaka embryogenesis. Advantages and potential disadvantages of the p-JRES approach are discussed.

PMID:
14550295
DOI:
10.1016/j.bbrc.2003.09.092
[Indexed for MEDLINE]

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