Abstract
We previously described the isolation of CDK4, representing the 3(')end of an unknown mRNA, using differential display [Biophys. Res. Commun. 232 (1997) 49]. To determine the identity of the CDK4 gene, full-length CDK4 cDNA clones were isolated from a diabetic kidney cDNA library. DNA sequencing revealed that CDK4 represents the rat beta defensin-1 gene (rBD-1). rBD-1 mRNA was detected in rat kidney, heart, lung, and skeletal muscle using RT-PCR. Using Northern blot analysis, elevated levels of the 0.5kb mRNA transcript were detected in the kidneys of 6-, 16-, 26-, and 40-week-old GK rats when compared with age-matched Wistar controls. A novel 2.0kb mRNA transcript was also detected in all kidneys examined from GK rats in none of the age-matched control kidneys. Biglycan and TGF-beta1 mRNAs were significantly up-regulated in kidneys of GK rats at 26 weeks compared to 16 and 6 weeks, showing that the kidneys of GK rats mimic the gene expression pattern described for human and experimental DN. These data suggest that overexpression of beta-defensin-1 mRNA may play a role in diabetic nephropathy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Amino Acid Sequence
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Animals
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Base Sequence
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Biglycan
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases / genetics
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Decorin
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Defensins / biosynthesis
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Defensins / genetics*
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Diabetic Nephropathies / genetics
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Diabetic Nephropathies / metabolism*
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Extracellular Matrix Proteins
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Kidney / chemistry
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Kidney / metabolism
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Molecular Sequence Data
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Proteoglycans / genetics
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Proteoglycans / metabolism
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Proto-Oncogene Proteins*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Rats
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Rats, Mutant Strains
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Rats, Wistar
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Tissue Distribution
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta1
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beta-Defensins / biosynthesis
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beta-Defensins / genetics*
Substances
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3' Untranslated Regions
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BGN protein, human
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Bgn protein, rat
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Biglycan
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DCN protein, human
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DEFB1 protein, human
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Dcn protein, rat
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Decorin
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Defb1 protein, rat
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Defensins
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Extracellular Matrix Proteins
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Proteoglycans
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Proto-Oncogene Proteins
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RNA, Messenger
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TGFB1 protein, human
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Tgfb1 protein, rat
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Transforming Growth Factor beta
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Transforming Growth Factor beta1
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beta-Defensins
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Cdk4 protein, rat
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases