Abstract
Adenylate cyclase deficient (cya) mutants of E. coli K-12 were found to be resistant to fosmidomycin, a specific inhibitor of the non-mevalonate pathway, just like to fosfomycin. E. coli glpT mutants were resistant to fosfomycin and also to fosmidomycin. This fact shows that fosmidomycin was transported inside via the glycerol-3-phosphate transporter, GlpT. DNA micro-array analysis showed that the transcription of glpT and other genes concerning glycerol utilization were highly dependent on the presence of cAMP.
MeSH terms
-
Adenylyl Cyclases / deficiency
-
Adenylyl Cyclases / genetics*
-
Anti-Bacterial Agents / metabolism
-
Anti-Bacterial Agents / pharmacology
-
Cyclic AMP / pharmacology
-
Drug Resistance, Microbial / genetics
-
Escherichia coli / drug effects*
-
Escherichia coli / enzymology
-
Escherichia coli / genetics*
-
Fosfomycin / analogs & derivatives*
-
Fosfomycin / chemistry
-
Fosfomycin / metabolism
-
Fosfomycin / pharmacology*
-
Gene Expression Profiling
-
Genotype
-
Microbial Sensitivity Tests
-
Mutation
-
Oligonucleotide Array Sequence Analysis
Substances
-
Anti-Bacterial Agents
-
Fosfomycin
-
fosmidomycin
-
Cyclic AMP
-
Adenylyl Cyclases