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Curr Microbiol. 2003 Aug;47(2):87-92.

2-ketogluconic acid production and phosphate solubilization by Enterobacter intermedium.

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Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture, Chonnam National University, Gwangju 500-757, Korea.


Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200-250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period.

[Indexed for MEDLINE]

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