Format

Send to

Choose Destination
Cell Motil Cytoskeleton. 2003 Oct;56(2):130-9.

A Lim protein involved in the progression of cytokinesis and regulation of the mitotic spindle.

Author information

1
University of Wisconsin-Madison, Department of Physiology, Madison, WI 53706, USA.

Abstract

DdLimE regulates cell motility and cytokinesis in Dictyostelium. To specify its function, we generated knock-out mutants and analyzed mitosis by marking the mitotic apparatus with GFP-alpha-tubulin. Characteristic of DdLimE-null cells is a late reversal of cytokinesis caused by backward movement of the incipient daughter cells. This process of "retro-cytokinesis" is accompanied by a delay in disassembly of the mitotic spindle. The length of interphase microtubules is increased and their depolymerization at prophase is impaired. These data indicate that DdLimE links the cortical actin network, where it is located, to the microtubule system, whose dynamics it regulates.

PMID:
14506710
DOI:
10.1002/cm.10139
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center