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Fertil Steril. 2003 Sep;80 Suppl 2:736-42.

Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human preimplantation embryos.

Author information

1
Reproductive Immunology Laboratory, Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California 94305, USA.

Abstract

OBJECTIVE:

To examine human embryos at various stages of preimplantation development for simultaneous expression of matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinases (TIMPs).

DESIGN:

mRNAs of specific MMPs and TIMPs were examined in single human embryos, at different stages of preimplantation development, by reverse transcription-polymerase chain reaction (RT-PCR). Single embryo immunohistochemistry was applied to examine the protein expression.

SETTING:

University-affiliated IVF-ET program.

PATIENT(S):

Couples, attending the university-affiliated IVF-ET program, electing to donate poor prognosis embryos with anomalous morphology.

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

The expression of MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 in preimplantation embryos.

RESULT(S):

The MMP-2 mRNA was expressed consistently during development from one-cell to blastocyst stage. The TIMP-1 and TIMP-3 mRNAs were detected in embryos at all stages; however, in the later preimplantation developmental stages, an increasing proportion of embryos expressing TIMP-1 and TIMP-3 mRNA were noted. The MMP-1, MMP-9, and TIMP-2 mRNAs were detected in only a minority of human embryos studied. Immunohistochemistry showed MMP-1 and TIMP-1 protein expression in preimplantation embryos.

CONCLUSION(S):

The existence of MMP and TIMP mRNA expression in human preimplantation embryos argues for a role for these metalloproteinases and their inhibitors during the process of implantation in humans.

PMID:
14505747
DOI:
10.1016/s0015-0282(03)00782-9
[Indexed for MEDLINE]

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