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Arch Microbiol. 2003 Nov;180(5):367-73. Epub 2003 Sep 20.

Conjugative plasmid pIP501 undergoes specific deletions after transfer from Lactococcus lactis to Oenococcus oeni.

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1
Departament de Microbiologia, Universitat de València, 46100-Burjassot, Valencia, Spain.

Abstract

Conjugal transfer of plasmids pIP501 and its derivative pVA797 from Lactococcus lactis to Oenococcus oeni was assayed by filter mating. Plasmid pIP501 was transferred to a number of O. oeni strains whereas a single transconjugant of O. oeni M42 was recovered when pVA797 was used. Physical analysis of the transconjugant plasmids revealed that pIP501 and pVA797 underwent extensive deletions in O. oeni that affected the tra region (conjugal transfer) and SegB region (stability). All derivatives showed segregational instability in O. oeni, but were stably maintained in L. lactis. These differences correlated with the different plasmid copy numbers and the extent of deletions within the SegB region.

PMID:
14504693
DOI:
10.1007/s00203-003-0599-3
[Indexed for MEDLINE]
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