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J Microbiol Methods. 2003 Oct;55(1):315-9.

A high-throughput capillary assay for bacterial chemotaxis.

Author information

1
The James Franck Institute and the Institute for Biophysical Dynamics, University of Chicago, 5640 S. Ellis Av., Chicago, IL 60637, USA.

Abstract

We present a high-throughput capillary assay in order to characterize the chemotactic response of the E. coli bacterium. We measure the number of organisms attracted into an array of 96 capillary tubes containing the attractant L-aspartate. The effect of bacterial concentration on the chemotactic response is reported. Such high-throughput assay can be used to characterize bacterial chemotaxis function of a wide range of biochemical parameters.

PMID:
14500024
DOI:
10.1016/s0167-7012(03)00112-x
[Indexed for MEDLINE]

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