Format

Send to

Choose Destination
Virology. 1992 Dec;191(2):837-45.

Complex intrapatient sequence variation in the V1 and V2 hypervariable regions of the HIV-1 gp 120 envelope sequence.

Author information

1
Laboratoire de R├ętrovirologie Mol├ęculaire, Institut Pasteur, Paris.

Abstract

Human immunodeficiency virus (HIV) quasi-species fluctuate both in time and in space. In general intrapatient variation is less extensive than interpatient variation. The V1 and V2 hypervariable regions of the envelope gene were analyzed for patient samples harboring highly divergent genomes in other loci. Proviral sequences were amplified by PCR, cloned, and sequenced. It was concluded that intrapatient variation may exceed interpatient variation but the presence of two clearly distinct populations was not confirmed. The env hypervariable regions appeared to be evolving independently of one another, cautioning against extrapolation of data, particularly from the V3 region. Virus typing and the assessment of HIV superinfection by genetic methods will prove difficult and experimental approaches will have to be carefully designed.

PMID:
1448927
DOI:
10.1016/0042-6822(92)90259-r
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center