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Peptides. 1992 Jul-Aug;13(4):671-5.

Insulin in the vitreous of the normal and streptozotocin-induced diabetic rat.

Author information

1
Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242.

Abstract

Insulin has been detected by ELISA in the vitreous of the normal and streptozotocin-diabetic rat at levels for both about 1% of those in serum. 131I-labeled insulin, administered to conscious rats via an indwelling cannula in the right atrium, was found to cross the blood-ocular barrier into the vitreous. Autoradiographic gel analysis showed the peptide was transferred as an intact molecule. Vitreous insulin levels reflected serum levels as seen in relatively constant vitreous-to-serum insulin ratios over a wide range of serum insulin concentrations. The rate of blood-to-vitreous passage of insulin was about the same in normal as in diabetic rats (fasting serum glucose greater than or equal to 21 mM). At least a portion of vitreous insulin is therefore of pancreatic origin, and retinal tissue in the normal and diabetic animal is thus accessible to circulating hormone. The blood-ocular barrier is unaltered in streptozotocin diabetes with regard to insulin passage.

PMID:
1437710
DOI:
10.1016/0196-9781(92)90171-x
[Indexed for MEDLINE]

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