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Nucleic Acids Res. 1992 Oct 25;20(20):5407-12.

DeoR repression at-a-distance only weakly responds to changes in interoperator separation and DNA topology.

Author information

1
Institute of Biological Chemistry B, University of Copenhagen, Denmark.

Abstract

The interoperator distance between a synthetic operator Os and the deoP2O2-galK fusion was varied between 46 and 176 bp. The repression of the deoP2 directed galK expression as a function of the interoperator distance (center-to-center) was measured in vivo in a single-copy system. The results show that the DeoR repressor efficiently can repress transcription at all the interoperator distances tested. The degree of repression depends very little on the spacing between the operators, however, a weak periodic dependency of 8-11 bp may exist.

PMID:
1437558
PMCID:
PMC334349
DOI:
10.1093/nar/20.20.5407
[Indexed for MEDLINE]
Free PMC Article

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