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Biotechniques. 1992 Nov;13(5):744-6, 749.

Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix.

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Department of Protein Chemistry, Symbicom AB, UMEA, Sweden.


A simple and sensitive method for the detection of glycoproteins and glycopeptides in solution and in polyacrylamide gels is described. This method combines the well-known periodic acid/Schiff stain with protein blotting. Compared with direct staining of a polyacrylamide gel, the sensitivity is considerably increased. Using a set of glycoproteins, we have found the sensitivity to be about 4 ng carbohydrate.

[Indexed for MEDLINE]

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