Format

Send to

Choose Destination
Arthritis Rheum. 1992 Sep;35(9):1068-74.

Signal transduction in Sjögren's syndrome T cells. Abnormalities associated with a newly described human A-type retrovirus.

Author information

1
Clinical Immunology Section, Audie L. Murphy Memorial Veterans Hospital, San Antonio, Texas.

Abstract

OBJECTIVE:

To study the effects of a novel A-type retrovirus, detected in cocultures of lip biopsy specimens from Sjögren's syndrome (SS) patients and a human T cell line, on the infected T cells.

METHODS:

Interleukin-2 (IL-2) and IL-6 secretion were measured by bioassay and enzyme-linked immunosorbent assay, respectively, in the infected and noninfected cell lines. Surface antigen expression was determined by flow cytometry, using monoclonal antibodies. Protein kinase C (PKC) activity was measured using an enzyme assay kit, and calcium mobilization was assessed with a fluorescent probe.

RESULTS:

Infected cells expressed less CD4 and IL-6 receptor, but more HLA-DR, compared with noninfected cells. Infected cells also produced less IL-2 and displayed reduced PKC activation and calcium mobilization. A similar defect in calcium mobilization was detected in T cells from SS patients.

CONCLUSION:

These data suggest a possible involvement of the newly described retrovirus in T cell abnormalities.

PMID:
1418023
DOI:
10.1002/art.1780350912
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center