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Antimicrob Agents Chemother. 1992 Aug;36(8):1619-25.

Innovative endpoint determination system for antifungal susceptibility testing of yeasts.

Author information

1
Department of Microbiology, Toronto Hospital, Ontario, Canada.

Abstract

Fungal infections and antifungal resistance are increasingly recognized. Antifungal susceptibility testing remains unstandardized, and a particularly important problem is endpoint determination. In this paper we propose the yeast metabolic reduction of the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetraz olium hydroxide (XTT) as a colorimetric endpoint which is quantitative and objective. Amphotericin B, fluorocytosine, and fluconazole dose-response curves were obtained, and a metabolic MIC could be determined by using precise criteria.

PMID:
1416843
PMCID:
PMC192019
DOI:
10.1128/aac.36.8.1619
[Indexed for MEDLINE]
Free PMC Article

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