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J Bacteriol. 1964 Apr;87:828-31.

PREFERENTIAL SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS IN THE PRESENCE OF 5-METHYL-TRYPTOPHAN.

Abstract

Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferentially synthesized alpha-amylase in the presence of 5-methyl-tryptophan (5-MT). 5-MT did not inhibit the formation of active alpha-amylase or the incorporation of proline-C(14) into alpha-amylase. In contrast, p-fluorophenylalanine inhibited the formation of active alpha-amylase by 92% and the incorporation of proline-C(14) into the enzyme by 95%. p-Fluorophenylalanine and 5-MT inhibited cellular protein synthesis, as measured by proline-C(14) incorporation, by 74 and 72%, respectively.

PMID:
14137620
PMCID:
PMC277100
[Indexed for MEDLINE]
Free PMC Article

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