Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA

J Bitinaite; Z Maneliene; S Menkevicius; S Klimasauskas; V Butkus; A Janulaitis

doi:10.1093/nar/20.19.4981

Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA

Nucleic Acids Res. 1992 Oct 11;20(19):4981-5. doi: 10.1093/nar/20.19.4981.

Authors

J Bitinaite¹, Z Maneliene, S Menkevicius, S Klimasauskas, V Butkus, A Janulaitis

Affiliation

¹ Institute of Biotechnology FERMENTAS, Vilnius, Lithuania.

Abstract

The specificity of three DNA methyltransferases M.Alw26I, M.Eco31I and M.Esp3I, isolated from Acinetobacter Iwoffi RFL26, Escherichia coli RFL31 and Hafnia alvei RFL3+, respectively, was determined. All the enzymes methylate both strands of asymmetric recognition sites yielding m5C in the top-strand and m6A in the bottom-strand, as below: 5'-GTm5CTC 5'-GGTm5CTC 5'-CGTm5CTC 3'-Cm6AGAG 3'-CCm6AGAG 3'-GCm6AGAG (M.Alw26I) (M.Eco31I) (M.Esp3I) They are the first members of type IIs methyltransferases that modify different types of nucleotides in the recognition sequence.

MeSH terms

Acinetobacter / enzymology*
Base Sequence
DNA Modification Methylases / metabolism*
DNA-Cytosine Methylases / metabolism*
Enterobacteriaceae / enzymology*
Escherichia coli / enzymology*
Kinetics
Molecular Sequence Data
Oligodeoxyribonucleotides / metabolism
Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*
Substrate Specificity

Substances

Oligodeoxyribonucleotides
DNA Modification Methylases
DNA modification methylase Alw26I
DNA modification methylase Eco31I
DNA modification methylase Esp3I
DNA-Cytosine Methylases
Site-Specific DNA-Methyltransferase (Adenine-Specific)