Determination of creatinine and purine derivatives in ruminants' urine by reversed-phase high-performance liquid chromatography

J Chromatogr. 1992 Aug 28;607(2):199-202. doi: 10.1016/0021-9673(92)87075-j.

Abstract

A procedure is described for the rapid and simultaneous determination of allantoin, creatinine, uric acid, hypoxanthine and xanthine in sheep urine. Separation was achieved on a Novapak C18 column under isocratic conditions. The mobile phase was potassium phosphate buffer (10 mM, pH 4.0). A flow-rate of 0.5 ml/min, detection at 218 nm and a column temperature of 25 degrees C were employed with a total analysis time of less than 15 min. Detection limits for allantoin, creatinine, uric acid, hypoxanthine and xanthine were 1.0, 0.5, 0.5, 0.5 and 0.2 micrograms/ml, respectively, at a signal-to-noise ratio of 3 in a 20-microliters injection volume of tenfold-diluted urine. This sensitivity permits the precise determination of these compounds in ruminants' urine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Creatinine / urine*
  • Indicators and Reagents
  • Purines / urine*
  • Sheep
  • Spectrophotometry, Ultraviolet

Substances

  • Indicators and Reagents
  • Purines
  • Creatinine