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Biotechniques. 1992 Sep;13(3):388-91.

Improved method for screening cDNA expression libraries for DNA-binding proteins.

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  • 1Department of Molecular Microbiology and Immunology, University of Missouri-Columbia 65212.


The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that probes labeled by the PCR are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated our efforts to isolate recombinant phage containing putative DNA-binding gene products that recognized a 246-base pair transcriptional enhancer region of Rous sarcoma virus long terminal repeat.

[PubMed - indexed for MEDLINE]
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