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Biotechniques. 1992 Aug;13(2):208-10.

UV absorption complicates PCR decontamination.

Author information

1
Duke University Medical Center, Durham, NC 27710.

Abstract

UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.

PMID:
1389153
[Indexed for MEDLINE]

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