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Nature. 1992 Oct 8;359(6395):500-5.

Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic.

Author information

1
Unité de Recherche Associée au Centre National de la Recherche Scientifique D1284, Institut Pasteur, Paris, France.

Abstract

Introduction by site-directed mutagenesis of three amino acids from the MII segment of glycine or gamma-aminobutyric acid (GABAA) receptors into the MII segment of alpha 7 nicotinic receptor was sufficient to convert a cation-selective channel into an anion-selective channel gated by acetylcholine. A critical mutation was the insertion of an uncharged residue at the amino-terminal end of MII, stressing the importance of protein geometrical constraints on ion selectivity.

PMID:
1383829
DOI:
10.1038/359500a0
[Indexed for MEDLINE]

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