Rapid effects of insulin on cyclic GMP location in an intact protozoan

L Kohidai; J Barsony; J Roth; S J Marx

doi:10.1007/BF01928167

Rapid effects of insulin on cyclic GMP location in an intact protozoan

Experientia. 1992 May 15;48(5):476-81. doi: 10.1007/BF01928167.

Authors

L Kohidai¹, J Barsony, J Roth, S J Marx

Affiliation

¹ Metabolic Diseases Branch, NIDDK, Bethesda, Maryland 20892.

PMID: 1376280
DOI: 10.1007/BF01928167

Abstract

We studied rapid changes in location of cyclic GMP in Tetrahymena pyriformis. Insulin caused cGMP localization in cilia and near the plasma membrane (0.5-1 min). Later (1-5 min) cGMP localization was diffuse in cytoplasm with perinuclear accentuation. Inactive insulin analogs did not elicit these changes.

MeSH terms

1-Methyl-3-isobutylxanthine / pharmacology
Animals
Cell Membrane / metabolism
Cilia / drug effects
Cilia / metabolism
Cyclic GMP / metabolism*
Cytoplasm / drug effects
Cytoplasm / metabolism
Insulin / analogs & derivatives
Insulin / pharmacology*
Nitroprusside / pharmacology
Tetrahymena pyriformis / drug effects
Tetrahymena pyriformis / metabolism*
Tetrahymena pyriformis / ultrastructure
Tissue Distribution

Substances

Insulin
Nitroprusside
Cyclic GMP
1-Methyl-3-isobutylxanthine