Abstract
Cultured rat forebrain astrocytes contained significant amounts of immunostainable heme oxygenase-1 (HO-1) isozyme, whereas HO-1 was undetectable in spontaneously transformed rat astroglial cells (ATs). HO-1 was inducible in both cell types by heat shock and by submicromolar amounts of H2O2. Inhibition of RNA synthesis with actinomycin D or protein synthesis with cycloheximide resulted in the rapid loss of immunostainable heme oxygenase in astrocytes. Analysis of the primary structure of heme oxygenase suggests that it is a PEST protein, i.e., targeted for rapid turnover.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Astrocytes / drug effects
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Astrocytes / enzymology*
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cell Line, Transformed
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Cycloheximide / pharmacology
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Dactinomycin / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation, Enzymologic
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Heat-Shock Proteins / biosynthesis
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / isolation & purification
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Heme Oxygenase (Decyclizing) / biosynthesis
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Heme Oxygenase (Decyclizing) / genetics*
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Heme Oxygenase (Decyclizing) / isolation & purification
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Hot Temperature*
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Hydrogen Peroxide / pharmacology*
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Isoenzymes / biosynthesis
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Isoenzymes / genetics*
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Isoenzymes / isolation & purification
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Molecular Sequence Data
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Oligonucleotides, Antisense
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Prosencephalon / enzymology*
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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Rats
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Transcription, Genetic / drug effects
Substances
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Heat-Shock Proteins
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Isoenzymes
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Oligonucleotides, Antisense
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RNA, Messenger
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Dactinomycin
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RNA
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Cycloheximide
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Hydrogen Peroxide
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Heme Oxygenase (Decyclizing)