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Nucleic Acids Res. 1992 Feb 25;20(4):847-50.

Processing in the 5' region of the pnp transcript facilitates the site-specific endonucleolytic cleavages of mRNA.

Author information

1
Department of Biotechnology, College of Agriculture, Ehime University, Japan.

Abstract

The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.

PMID:
1371867
PMCID:
PMC312027
DOI:
10.1093/nar/20.4.847
[Indexed for MEDLINE]
Free PMC Article

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