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Mutat Res. 1992 Mar;281(3):221-5.

Construction of a umuDC operon substitution mutation in Escherichia coli.

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Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892.


Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.

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