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Lett Appl Microbiol. 1991 Jul;13(1):21-4.

DNA recovery and direct detection of Tn5 sequences from soil.

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Department of Genetics, University of Bayreuth, Germany.


Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.

[Indexed for MEDLINE]

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